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Refolding of most proteins is a self-assembly process that occurs spontaneously under the appropriate conditions in 10−1–103 s.Each molecule is associated with many types of energies of which the most important are: Etotal = Eelectronic + Evibration + Erotation + Etranslation + Eelectron spin orientation + Enuclear spin orientation Each of these energies is quantized into energy levels that are characteristic states of the molecule.
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